This database provides qRT PCR primers for 99.96% human RefSeq sequences.
For 99% of intron-bearing genes, the PCR product will cross an exon-exon border which overlaps one of the largest introns.
All primers have annealing temperatures approximately 60C.
Please input RefSeq ID (such as NM_xxxxxx) or HUGO gene name (such as JUN) here:
Wenwu Cui PhD
Laboratory of Receptor Biology and Gene Expression
National Cancer Institute
National Institutes of Health